| EST name | EST sequence |
| gi|84418282|gb|DW252880.1|DW252880 | TTTTTTTTTTTTTTTTTTCAGGATCTTTGCGAGATGCCCTGCTTTAACGGCTTCATACCACAGGAACTGCCACAGGAGGA AGACCACTTGGGCGACACGGGGTAGGACCTGGAACAGCTGTAGCCGTTATCCCCCCTCTGTGAGCAGGGAGAGCTCATCA TATGGGGAGGCGATTGCTGAGACATCTGGCGAGGGCATATTGCGGCCATGGATGAAGTCTGGATGGTCAATAGTTTGCCA GTTATTGGTGGTGGTTGTTGGCTGGGCGACATTCCACACGGCGAGAGCGGGGGAGACATACAGCTGCAGTTGCAGCCGCA CGACAGGGGCGGCGAAGGTGGAGGTGGCACACATTGTTGCTGTTGAGACTGCTGCTGCTGCTGCTGTTGCTTGTAACTGG GAGGAGGGCTGTCCAGACTGACCCCTGCTCGAGCCAATTCTTCGCTGCTGTGGCTGCGTTCGTGCTTCTTCATGTGTACT CTCTGTGGGAAGCTTTTGTCACAAACGACGCACGTAAAGGGTTTTTCACCTGAATGTGTTCGCTGATGGATGGTCAGGTG GGCCTTCTGAGCGAATGCTTTATCGCATTGGAAACATGAGTACGGTCTCTCACCTGTGTGAGTTCTGAGATGATTCCGCA GGTGCGATCGTTGTGCAAACGACTTGCCGCANAAAGTGCAATGAAATGGCCGCTCCCCAGTGTGCACGCGTTCATGGTTA TTCANATGGTCACGCTGGGCAAAAGCTTCGGACATATGAGAACTGTACGGTTTTCTCCGGAGTGATGCGCGA |
Most similar to sp|P17030|ZNF25_HUMAN
>BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|Q9AWM9|RPF2_ORYSJ Ribosome production factor 2 homolog OS=Oryza
sativa subsp. japonica GN=Os01g0513800 PE=2 SV=1
Length = 310
Score = 160 bits (405), Expect(2) = 6e-62
Identities = 77/116 (66%), Positives = 89/116 (76%)
Frame = +1
Query: 13 TYDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXX 192
TYDHHIYDLVEVGVEN+KS+ S YDKK+AP +GSKPFFAFIGE F
Sbjct: 109 TYDHHIYDLVEVGVENYKSIESYVYDKKLAPKLGSKPFFAFIGEHFESVEELKHLKEVLL 168
Query: 193 DLFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAP 360
DLF+G+VV NLNLAG+DRV+VCTA+S T V HCALRLK+SGT +PR+ELVEV P
Sbjct: 169 DLFKGEVVENLNLAGVDRVFVCTAISPTTVYMMHCALRLKRSGTSIPRIELVEVGP 224
Score = 99.0 bits (245), Expect(2) = 6e-62
Identities = 55/94 (58%), Positives = 63/94 (67%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIYVPDQQVGSAS 533
GPSMDLVVRRHR P E LKKEA K KK KNV+KD + GK+GK+Y+PDQQ+ S
Sbjct: 223 GPSMDLVVRRHRYPVESLKKEAMKTADHA--KKMKNVTKDPVHGKLGKVYIPDQQIAKMS 280
Query: 534 LPFTPKGVKRERREAKAKGGDNKHAEKKQKQNDD 635
L KG+KRERREAK NK KKQK N +
Sbjct: 281 LSNDVKGLKRERREAK----KNKDHSKKQKINPE 310
>sp|Q9LUG5|RPF2_ARATH Ribosome production factor 2 homolog
OS=Arabidopsis thaliana GN=At3g23620 PE=2 SV=1
Length = 314
Score = 155 bits (393), Expect(2) = 4e-58
Identities = 73/115 (63%), Positives = 86/115 (74%)
Frame = +1
Query: 16 YDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXXD 195
YDH +YDL+EVG+ENFKS+ + SYDKK AP G+KPF FIGEGF D
Sbjct: 109 YDHQVYDLIEVGIENFKSLRAFSYDKKFAPHEGTKPFICFIGEGFENVSELKHLKEVLTD 168
Query: 196 LFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAP 360
LFRG+VV NLNL GLDR YVC+A+S TKV THCAL+LKKSG++VPR+ELVEV P
Sbjct: 169 LFRGEVVDNLNLTGLDRAYVCSAISPTKVFLTHCALKLKKSGSIVPRMELVEVGP 223
Score = 90.9 bits (224), Expect(2) = 4e-58
Identities = 46/93 (49%), Positives = 64/93 (68%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIYVPDQQVGSAS 533
GPSMDLV+RR+RLP++ L KEA + + KKKEKNV +DA+ GK GKIY+PDQ++
Sbjct: 222 GPSMDLVIRRNRLPNDSLMKEAMRTSKDKPKKKEKNVDQDAVLGKTGKIYMPDQKLKEMK 281
Query: 534 LPFTPKGVKRERREAKAKGGDNKHAEKKQKQND 632
L KG KRER++AK K + A+K + ++
Sbjct: 282 LFDKSKGSKRERKDAKLKHKEETVAKKMKVSSE 314
>sp|Q2YDN6|RPF2_BOVIN Ribosome production factor 2 homolog OS=Bos
taurus GN=RPF2 PE=2 SV=1
Length = 306
Score = 76.6 bits (187), Expect(2) = 5e-24
Identities = 44/115 (38%), Positives = 60/115 (52%)
Frame = +1
Query: 16 YDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXXD 195
YD+H+ D++E+G+E F S+ K G+KP F G+ F D
Sbjct: 109 YDYHVLDIIELGIEKFVSLKDIKNSKCPE---GTKPMLIFAGDDFDVTEDYRRLKSLLID 165
Query: 196 LFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAP 360
FRG VSN+ LAGL+ V TA++ K+ F L LKKSG PR+EL E+ P
Sbjct: 166 FFRGPTVSNIRLAGLEYVLHFTALNG-KIYFRSYKLLLKKSGCRTPRIELEEMGP 219
Score = 55.8 bits (133), Expect(2) = 5e-24
Identities = 34/92 (36%), Positives = 51/92 (55%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIYVPDQQVGSAS 533
GPS+DLV+RR L + L K + K+P L KK+KN+S D G+I++ Q +
Sbjct: 218 GPSLDLVLRRTHLASDDLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQ 277
Query: 534 LPFTPKGVKRERREAKAKGGDNKHAEKKQKQN 629
KG+K+ E KA+ +NK K+ K+N
Sbjct: 278 TR-KMKGLKKRPAERKAEDEENK--SKRIKKN 306
>sp|Q54DJ0|RPF2_DICDI Ribosome production factor 2 homolog
OS=Dictyostelium discoideum GN=bxdc1 PE=3 SV=1
Length = 307
Score = 82.0 bits (201), Expect(2) = 4e-23
Identities = 42/117 (35%), Positives = 64/117 (54%)
Frame = +1
Query: 16 YDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXXD 195
+D+ ++D++E+G+ NFKS+ D +G+KP F F G F D
Sbjct: 114 FDNAVFDMIEMGISNFKSIKEFGAD---GSAVGNKPMFIFQGNEFENNETVKKVGNLFLD 170
Query: 196 LFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAPQW 366
+FRG++V +NL GLD V + TAV K+LF H SG+ +P+V+L EV P +
Sbjct: 171 MFRGRLVEYINLVGLDHVVIMTAVDD-KILFRHYTTIFSPSGSKIPKVDLKEVGPSF 226
Score = 47.4 bits (111), Expect(2) = 4e-23
Identities = 20/50 (40%), Positives = 31/50 (62%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIY 503
GPS D+ +RR +L L+KEA+K+ ++ K +KNV DA+ G +Y
Sbjct: 223 GPSFDITIRRTKLASADLEKEAYKVHKSVVPKAKKNVETDALGQTFGTVY 272
>sp|Q9H7B2|RPF2_HUMAN Ribosome production factor 2 homolog OS=Homo
sapiens GN=RPF2 PE=1 SV=2
Length = 306
Score = 79.0 bits (193), Expect(2) = 4e-23
Identities = 45/115 (39%), Positives = 61/115 (53%)
Frame = +1
Query: 16 YDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXXD 195
YD+H+ D++E+G+ENF S+ K G+KP F G+ F D
Sbjct: 109 YDYHVLDMIELGIENFVSLKDIKNSKCPE---GTKPMLIFAGDDFDVTEDYRRLKSLLID 165
Query: 196 LFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAP 360
FRG VSN+ LAGL+ V TA++ K+ F L LKKSG PR+EL E+ P
Sbjct: 166 FFRGPTVSNIRLAGLEYVLHFTALNG-KIYFRSYKLLLKKSGCRTPRIELEEMGP 219
Score = 50.4 bits (119), Expect(2) = 4e-23
Identities = 31/92 (33%), Positives = 49/92 (53%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIYVPDQQVGSAS 533
GPS+DLV+RR L + L K + K+P L KK+KN+S D G+I++ Q +
Sbjct: 218 GPSLDLVLRRTHLASDDLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQ 277
Query: 534 LPFTPKGVKRERREAKAKGGDNKHAEKKQKQN 629
T K ++R A+ D++ K+ K+N
Sbjct: 278 ---TRKMKGLKKRPAERITEDHEKKSKRIKKN 306
>sp|Q9JJ80|RPF2_MOUSE Ribosome production factor 2 homolog OS=Mus
musculus GN=Rpf2 PE=2 SV=2
Length = 306
Score = 76.6 bits (187), Expect(2) = 2e-22
Identities = 45/115 (39%), Positives = 60/115 (52%)
Frame = +1
Query: 16 YDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFXXXXXXXXXXXXXXD 195
YD+H+ D++E+G+E F S+ K G+KP F G+ F D
Sbjct: 109 YDYHVLDMIELGIEKFVSLKDIKTSKCPE---GTKPMLIFAGDDFDVTEDFRRLKNLLID 165
Query: 196 LFRGQVVSNLNLAGLDRVYVCTAVSSTKVLFTHCALRLKKSGTVVPRVELVEVAP 360
FRG VSN+ LAGL+ V TA++ KV F L LKKSG PR+EL E+ P
Sbjct: 166 FFRGPTVSNVRLAGLEYVLHFTALNG-KVYFRSYKLLLKKSGCRTPRIELEEMGP 219
Score = 50.4 bits (119), Expect(2) = 2e-22
Identities = 31/92 (33%), Positives = 47/92 (51%)
Frame = +3
Query: 354 GPSMDLVVRRHRLPDEGLKKEAHKIPHELIKKKEKNVSKDAIAGKIGKIYVPDQQVGSAS 533
GPS+DLV+RR L + L K + K+P L KK KN+S+D G+I++ Q +
Sbjct: 218 GPSLDLVMRRTHLASDDLYKLSMKVPKALKPKKRKNISQDTFGTTFGRIHMQKQDLSKLQ 277
Query: 534 LPFTPKGVKRERREAKAKGGDNKHAEKKQKQN 629
T K ++R A+ D K+ K+N
Sbjct: 278 ---TRKMKGLKKRPAENGVDDQGKKSKRIKKN 306
BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|P17030|ZNF25_HUMAN Zinc finger protein 25 OS=Homo sapiens
GN=ZNF25 PE=2 SV=2
Length = 456
Score = 140 bits (354), Expect = 8e-33
Identities = 59/119 (49%), Positives = 81/119 (68%), Gaps = 1/119 (0%)
Frame = -2
Query: 791 RASLRRKPYSSHMSE-AFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHT 615
R KPY + E +F Q+ H H++ HTGE+PF CT CGK F +++L H +THT
Sbjct: 194 RTHAGEKPYECNQCEKSFYQKPHLTEHQKTHTGEKPFECTECGKFFYVKAYLMVHQKTHT 253
Query: 614 GERPYSCFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
GE+PY C +C KAF+QK+HLT+HQR H+GEKP+ C C K F + H+K H+RSH+ E+
Sbjct: 254 GEKPYECKECGKAFSQKSHLTVHQRMHTGEKPYKCKECGKFFSRNSHLKTHQRSHTGEK 312
Score = 136 bits (343), Expect = 1e-31
Identities = 57/113 (50%), Positives = 79/113 (69%), Gaps = 1/113 (0%)
Frame = -2
Query: 773 KPYS-SHMSEAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHTGERPYS 597
KPY +AF+Q+ H H+R+HTGE+P+ C CGK F++ SHL+ H R+HTGE+PY
Sbjct: 256 KPYECKECGKAFSQKSHLTVHQRMHTGEKPYKCKECGKFFSRNSHLKTHQRSHTGEKPYE 315
Query: 596 CFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
C +C K F QK+ LT+HQRTH+GEKPF C C K+F + + KH+R H+ E+
Sbjct: 316 CKECRKCFYQKSALTVHQRTHTGEKPFECNKCGKTFYYKSDLTKHQRKHTGEK 368
Score = 125 bits (314), Expect = 3e-28
Identities = 54/121 (44%), Positives = 78/121 (64%), Gaps = 1/121 (0%)
Frame = -2
Query: 791 RASLRRKPYSSHM-SEAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHT 615
R KP+ + + F + H+R HTGE+P+ CT CGKSFA S LR H RTHT
Sbjct: 334 RTHTGEKPFECNKCGKTFYYKSDLTKHQRKHTGEKPYECTECGKSFAVNSVLRLHQRTHT 393
Query: 614 GERPYSCFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEEL 435
GE+PY+C +C K+F+QK+H IHQR H+GEKP+ C C ++F Q+ + H+++H+ +
Sbjct: 394 GEKPYACKECGKSFSQKSHFIIHQRKHTGEKPYECQECGETFIQKSQLTAHQKTHTKKRN 453
Query: 434 A 432
A
Sbjct: 454 A 454
Score = 125 bits (313), Expect = 4e-28
Identities = 55/119 (46%), Positives = 73/119 (61%), Gaps = 1/119 (0%)
Frame = -2
Query: 791 RASLRRKPYS-SHMSEAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHT 615
R+ KPY + F Q+ H+R HTGE+PF C CGK+F +S L H R HT
Sbjct: 306 RSHTGEKPYECKECRKCFYQKSALTVHQRTHTGEKPFECNKCGKTFYYKSDLTKHQRKHT 365
Query: 614 GERPYSCFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
GE+PY C +C K+FA + L +HQRTH+GEKP+ C C KSF Q+ H H+R H+ E+
Sbjct: 366 GEKPYECTECGKSFAVNSVLRLHQRTHTGEKPYACKECGKSFSQKSHFIIHQRKHTGEK 424
Score = 118 bits (295), Expect = 5e-26
Identities = 50/115 (43%), Positives = 70/115 (60%), Gaps = 1/115 (0%)
Frame = -2
Query: 779 RRKPYS-SHMSEAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHTGERP 603
R K Y + F + H R H GE+P+ C C KSF Q+ HL H +THTGE+P
Sbjct: 170 RDKTYECKECKKIFYHLSSLSRHLRTHAGEKPYECNQCEKSFYQKPHLTEHQKTHTGEKP 229
Query: 602 YSCFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
+ C +C K F KA+L +HQ+TH+GEKP+ C C K+F Q+ H+ H+R H+ E+
Sbjct: 230 FECTECGKFFYVKAYLMVHQKTHTGEKPYECKECGKAFSQKSHLTVHQRMHTGEK 284
Score = 117 bits (292), Expect = 1e-25
Identities = 51/113 (45%), Positives = 70/113 (61%), Gaps = 1/113 (0%)
Frame = -2
Query: 773 KPYS-SHMSEAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHTGERPYS 597
KPY + F++ H H+R HTGE+P+ C C K F Q+S L H RTHTGE+P+
Sbjct: 284 KPYKCKECGKFFSRNSHLKTHQRSHTGEKPYECKECRKCFYQKSALTVHQRTHTGEKPFE 343
Query: 596 CFQCDKAFAQKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
C +C K F K+ LT HQR H+GEKP+ C C KSF ++ H+R+H+ E+
Sbjct: 344 CNKCGKTFYYKSDLTKHQRKHTGEKPYECTECGKSFAVNSVLRLHQRTHTGEK 396
Score = 113 bits (283), Expect = 1e-24
Identities = 45/104 (43%), Positives = 69/104 (66%)
Frame = -2
Query: 749 EAFAQRDHXNNHERVHTGERPFHCTXCGKSFAQRSHLRNHLRTHTGERPYSCFQCDKAFA 570
++F++ + H+++HT ++ + C C K F S L HLRTH GE+PY C QC+K+F
Sbjct: 153 KSFSKNEDLIRHQKIHTRDKTYECKECKKIFYHLSSLSRHLRTHAGEKPYECNQCEKSFY 212
Query: 569 QKAHLTIHQRTHSGEKPFTCVVCDKSFPQRVHMKKHERSHSSEE 438
QK HLT HQ+TH+GEKPF C C K F + ++ H+++H+ E+
Sbjct: 213 QKPHLTEHQKTHTGEKPFECTECGKFFYVKAYLMVHQKTHTGEK 256